Sample product 2

Sample product 2

£298

دسته: , , , ,

Contact your RoosterBio representative for information on cGMP formats of RoosterReplenish-MSC.

RoosterReplenishTM-MSC-XF is a concentrated bioreactor feed for use in a fed-batch bioreactor process, enhancing media productivity to drive a radically cost-effective process that is less labor-intensive in small- and large-scale expansions of hMSCs in 3D bioreactors. RoosterReplenishTM-MSC-XF is designed to achieve high cell growth in microcarrier-based suspension cultures when paired with our xeno-free (XF) hMSCs (derived from multiple tissue types) and paired bioprocess media (RoosterNourish™-MSC-XF). Using RoosterNourish™-MSC-XF and RoosterReplenishTM-MSC-XF in a fed-batch system not only increases culture yield, it also minimizes the time required for media preparation, eliminates media exchange, and reduces the contamination risk associated with in-process manipulation. RoosterReplenishTM-MSC-XF boosts media productivity (millions of cells produced per Liter of media consumed) by up to 70%. Each 10 mL bottle of RoosterReplenishTM-MSC-XF can be used to supplement 500 mL of RoosterNourishTM-MSC-XF, with analogous version available for cGMP manufacturing.

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Hi
Here you can see some frequently asked questions or contact us!

What passage are your cells?

Our human mesenchymal stem/stromal cells (hMSCs) are provided at a population doubling level (PDL) of 8-10 post-mononuclear isolation (Bone Marrow; Equivalent Passage 2) or 14-18 post-isolation from the perivascular region of Wharton’s Jelly (Umbilical Cord; Equivalent Passage 3). Due to variabilities of seeding and harvest densities we characterize our MSC “True Age” in terms of their PDL.

How often should I feed your cells?

A: There is no need to feed cells between passages. Our media is engineered to be a batch culture media, where no media exchange or feed is necessary when following our suggested protocols.

Are your vials sold from pooled donors?

No, we do not pool donors at any time during our production. Master Cell Banks (MCBs) are created from each donor source. Working Cell Banks (WCBs) are produced from our MCBs for general product sales.

What characterization do you provide for your hMSCs?

We characterize our hMSCs by following ISCT criteria: cell identity (surface marker expression), functional potency (angiogenic cytokine and IDO secretion), and trilineage differentiation (adipo-, osteo-, and chondrogenesis). This information is provided to allow customers to choose the optimal hMSC tissue type and donor for their specific application or target indication.